All serums are always applied after cleansing and toning, but first before any moisturizers, treatments, or other anti-aging products. This product can be used day or night. Fruit blended anti-oxidants combined with anti-aging peptides provide protection and free radical fighting power. This serum is an extremely high performing anti-oxidant. It far exceeds the recommended anti-oxidant value. A rare, Swiss apple stem cell ingredient helps protect the skin stem cells from aging. It is a hard working free radical scavenger preventing skin cell damage.